Yeast hexokinase mutants.
نویسندگان
چکیده
منابع مشابه
Cloning of genes that complement yeast hexokinase and glucokinase mutants.
Genes complementing the glucose-negative fructose-negative Saccharomyces cerevisiae triple mutant strain (hxkl hxk2 glk1), which lacks hexokinase PI, hexokinase PII, and glucokinase, were obtained from a pool of yeast DNA in the multicopy plasmid YEp13.
متن کاملActivators of yeast hexokinase.
The rate of the ATP:glucose transphosphorylase reaction catalyzed by yeast hexokinase, isozyme PII, is inhibited by lowering the pH of the reaction below 7.0, especially at suboptimal concentrations of ATP. This effect of acidity is largely overcome by activators such as orthophosphate, citrate, malate, 3-phosphoglycerate, and riboside triphosphates. Thus, in the acid range, ATP appears to serv...
متن کاملActivation of Yeast Hexokinase PI1
Activation of yeast hexokinase PI1 at low pH by citrate or ATP has been shown to occur at the monomer level, without requiring association to the dimer level. Thus, the previous suggestion (Steitz, T. A., Anderson, W. F., Fletterick, R. G., and Anderson, C. M. (1977) J. Biol. Chem. 252, 4494-4500) that anionic activators of hexokinase, such as citrate and ATP, act by binding to the intersubunit...
متن کاملKinetic studies of yeast hexokinase.
The mechanism of calf brain hexokinase has recently been studied in our laboratory (1). It was concluded from kinetic experiments that adenosine diphosphate (ADP) dissociates from the enzyme before addition of substrate glucose. This hypothesis was supported by kinetic studies of n-mannose, ADP, and glucose g-phosphate inhibition. The experiments appear to lend credence to theories in which an ...
متن کاملReversible inactivation and dissociation of yeast hexokinase.
Recent’ work on the reactivation and reconstitution of several denatured enzymes has provided increasing understanding of the process of reversible protein denaturation. Studies on ribonuclease by Anfinsen et al. (1) and White (2) have convincingly demonstrated that under favorable conditions a complet,ely denatured protein can resume the active three-dimensional configuration of the native mol...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)40181-5